PE-Cyanine5 Anti-Human/Mouse CD45R (B220) (RA3-6B2)
The RA3-6B2 antibody reacts with the human and mouse CD45 isoform known as CD45R, or B220, a protein tyrosine phosphatase of ≥ 220 kDa. CD45 is one of the most abundant hematopoietic markers, and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). Various isoforms are generated and expressed in cell-specific patterns, all critical for leukocyte function. In mouse, the CD45R/B220 isoform is predominantly found on B cells, at varying levels on all stages from pro-B cells to activated B cells, and may also be detected on certain T cell and NK cell subsets. It is of note that B220 is not similarly expressed on human B cells, where it appears to be differentiation-specific and therefore expressed on only some B cell subsets. Other forms of CD45 with restricted cellular expression include CD45RA, CD45RB, CD45RO and several others.
The RA3-6B2 antibody is one of the most consistently used leukocyte markers for B cells, T cell subsets and NK cell subsets in human and mouse. This antibody has also been reported as cross-reactive with feline CD45R/B220.
| Name | PE-Cyanine5 Anti-Human/Mouse CD45R (B220) (RA3-6B2) |
|---|---|
| Cat. No. | 55-0452 |
| Alternative Names | Ly-5, Lyt-4, T200 |
| Gene ID | 19264 |
| Clone | RA3-6B2 |
| Isotype | Rat IgG2a, κ |
| Reactivity | Human, Mouse |
| Cross Reactivity | Feline |
| Format | PE-Cyanine5 |
| Application | Flow Cytometry |
PE-Cyanine5 Anti-Human/Mouse CD45R (B220) (RA3-6B2)
The RA3-6B2 antibody reacts with the human and mouse CD45 isoform known as CD45R, or B220, a protein tyrosine phosphatase of ≥ 220 kDa. CD45 is one of the most abundant hematopoietic markers, and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). Various isoforms are generated and expressed in cell-specific patterns, all critical for leukocyte function. In mouse, the CD45R/B220 isoform is predominantly found on B cells, at varying levels on all stages from pro-B cells to activated B cells, and may also be detected on certain T cell and NK cell subsets. It is of note that B220 is not similarly expressed on human B cells, where it appears to be differentiation-specific and therefore expressed on only some B cell subsets. Other forms of CD45 with restricted cellular expression include CD45RA, CD45RB, CD45RO and several others.
The RA3-6B2 antibody is one of the most consistently used leukocyte markers for B cells, T cell subsets and NK cell subsets in human and mouse. This antibody has also been reported as cross-reactive with feline CD45R/B220.
| Name | PE-Cyanine5 Anti-Human/Mouse CD45R (B220) (RA3-6B2) |
|---|---|
| Cat. No. | 55-0452 |
| Alternative Names | Ly-5, Lyt-4, T200 |
| Gene ID | 19264 |
| Clone | RA3-6B2 |
| Isotype | Rat IgG2a, κ |
| Reactivity | Human, Mouse |
| Cross Reactivity | Feline |
| Format | PE-Cyanine5 |
| Application | Flow Cytometry |
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Description
The RA3-6B2 antibody reacts with the human and mouse CD45 isoform known as CD45R, or B220, a protein tyrosine phosphatase of ≥ 220 kDa. CD45 is one of the most abundant hematopoietic markers, and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). Various isoforms are generated and expressed in cell-specific patterns, all critical for leukocyte function. In mouse, the CD45R/B220 isoform is predominantly found on B cells, at varying levels on all stages from pro-B cells to activated B cells, and may also be detected on certain T cell and NK cell subsets. It is of note that B220 is not similarly expressed on human B cells, where it appears to be differentiation-specific and therefore expressed on only some B cell subsets. Other forms of CD45 with restricted cellular expression include CD45RA, CD45RB, CD45RO and several others.
The RA3-6B2 antibody is one of the most consistently used leukocyte markers for B cells, T cell subsets and NK cell subsets in human and mouse. This antibody has also been reported as cross-reactive with feline CD45R/B220.
| Name | PE-Cyanine5 Anti-Human/Mouse CD45R (B220) (RA3-6B2) |
|---|---|
| Cat. No. | 55-0452 |
| Alternative Names | Ly-5, Lyt-4, T200 |
| Gene ID | 19264 |
| Clone | RA3-6B2 |
| Isotype | Rat IgG2a, κ |
| Reactivity | Human, Mouse |
| Cross Reactivity | Feline |
| Format | PE-Cyanine5 |
| Application | Flow Cytometry |
