CFSE
CFSE is a non-fluorescent molecule that easily diffuses across cell membranes. Inside the cell, acetate groups are cleaved by intracellular esterases yielding a fluorescent molecule whose succinimidyl ester group covalently interacts with primary amines of intracellular proteins. CFSE is compatible with standard intracellular staining protocols using aldehyde fixation and saponin-based permeabilization. CFSE is widely used to track cell division and to monitor cell migration in vivo. CFSE can be detected using standard fluorescein filter sets by fluorescence microscopy or flow cytometry.
Download the CFSE Labeling Protocol.
Recent Publications:
Meng YM, Jiang X, Zhao X, Meng Q, Wu S, Chen Y, Kong X, Qiu X, Su L, Huang C, Wang M, Liu C, Wong PP. Hexokinase 2-driven glycolysis in pericytes activates their contractility leading to tumor blood vessel abnormalities. Nat Commun. 2021 Oct 14;12(1):6011. doi: 10.1038/s41467-021-26259-y. PMID: 34650057.
» View on Nature Communications
Sun Y, Lu D, Yin Y, Song J, Liu Y, Hao W, Qi F, Zhang G, Zhang X, Liu L, Lin Z, Liang H, Zhao X, Jin Y, Yin Y. PTENα functions as an immune suppressor and promotes immune resistance in PTEN-mutant cancer. Nat Commun. 2021 Aug 26;12(1):5147. doi: 10.1038/s41467-021-25417-6. PMID: 34446716.
» View on Nature Communications
Do P, Perdue LA, Chyong A, Hunter R, Dougan J, Henry CJ, Porter CC, Dreaden EC. Rapid Assembly and Screening of Multivalent Immune Cell-Redirecting Therapies for Leukemia. ACS Comb Sci. 2020 Oct 12;22(10):533-541. doi: 10.1021/acscombsci.0c00081. Epub 2020 Aug 18. PMID: 32786324.
DomĂnguez-Romero AN, MartĂnez-CortĂ©s F, MunguĂa ME, Odales J, Gevorkian G, Manoutcharian K. Generation of multiepitope cancer vaccines based on large combinatorial libraries of survivin-derived mutant epitopes [published online ahead of print, 2020 Jul 3]. Immunology. 2020;10.1111/imm.13233. doi:10.1111/imm.13233.
Brincks E. L., Adams J., Wang L., Turner B., Marcinowicz A., Ke J., Essmann M., Mautino L. M., Allen C. Van, Kumar S., Vahanian N., Link C., Mautino M. R. et al Indoximod opposes the immunosuppressive effects mediated by IDO and TDO via modulation of AhR function and activation of mTORC1. Oncotarget. 2020; 11: 2438-2461.
Rana PS, Mudrak NJ, Lopez R, Lynn M, Kershner L and Model MA. 2017. Phase separation in necrotic cells. Biochem Biophys Res Commun. doi: 10.1016/j.bbrc.2017.08.123.
Murphy S, Patrick K, Thoner T, Edwards RW and Gubbels Bupp MR. 2016. T cell up-regulation of CD127 is associated with reductions in the homeostatic set point of the peripheral Tcell pool during malnourishment. Biochemistry and Biophysics Reports. doi:10.1016/j.bbrep.2016.06.006
Barwick BG, Scharer CD, Bally APR and Boss JM. 2016. Plasma Cell Differentiation is Coupled to Division-Dependent DNA Hypomethylation and Gene Regulation. Nat Immunol. 17(10):1216-25
| Name | CFSE |
|---|---|
| Cat. No. | 13-0850 |
| Protocol | CFSE Labeling Protocol |
| Excitation Laser | Blue (488 nm) |
| Emission (nm) | 521 |
| Formulation | lyophilized (500 ug) |
| Application | Flow Cytometry |
CFSE
CFSE is a non-fluorescent molecule that easily diffuses across cell membranes. Inside the cell, acetate groups are cleaved by intracellular esterases yielding a fluorescent molecule whose succinimidyl ester group covalently interacts with primary amines of intracellular proteins. CFSE is compatible with standard intracellular staining protocols using aldehyde fixation and saponin-based permeabilization. CFSE is widely used to track cell division and to monitor cell migration in vivo. CFSE can be detected using standard fluorescein filter sets by fluorescence microscopy or flow cytometry.
Download the CFSE Labeling Protocol.
Recent Publications:
Meng YM, Jiang X, Zhao X, Meng Q, Wu S, Chen Y, Kong X, Qiu X, Su L, Huang C, Wang M, Liu C, Wong PP. Hexokinase 2-driven glycolysis in pericytes activates their contractility leading to tumor blood vessel abnormalities. Nat Commun. 2021 Oct 14;12(1):6011. doi: 10.1038/s41467-021-26259-y. PMID: 34650057.
» View on Nature Communications
Sun Y, Lu D, Yin Y, Song J, Liu Y, Hao W, Qi F, Zhang G, Zhang X, Liu L, Lin Z, Liang H, Zhao X, Jin Y, Yin Y. PTENα functions as an immune suppressor and promotes immune resistance in PTEN-mutant cancer. Nat Commun. 2021 Aug 26;12(1):5147. doi: 10.1038/s41467-021-25417-6. PMID: 34446716.
» View on Nature Communications
Do P, Perdue LA, Chyong A, Hunter R, Dougan J, Henry CJ, Porter CC, Dreaden EC. Rapid Assembly and Screening of Multivalent Immune Cell-Redirecting Therapies for Leukemia. ACS Comb Sci. 2020 Oct 12;22(10):533-541. doi: 10.1021/acscombsci.0c00081. Epub 2020 Aug 18. PMID: 32786324.
DomĂnguez-Romero AN, MartĂnez-CortĂ©s F, MunguĂa ME, Odales J, Gevorkian G, Manoutcharian K. Generation of multiepitope cancer vaccines based on large combinatorial libraries of survivin-derived mutant epitopes [published online ahead of print, 2020 Jul 3]. Immunology. 2020;10.1111/imm.13233. doi:10.1111/imm.13233.
Brincks E. L., Adams J., Wang L., Turner B., Marcinowicz A., Ke J., Essmann M., Mautino L. M., Allen C. Van, Kumar S., Vahanian N., Link C., Mautino M. R. et al Indoximod opposes the immunosuppressive effects mediated by IDO and TDO via modulation of AhR function and activation of mTORC1. Oncotarget. 2020; 11: 2438-2461.
Rana PS, Mudrak NJ, Lopez R, Lynn M, Kershner L and Model MA. 2017. Phase separation in necrotic cells. Biochem Biophys Res Commun. doi: 10.1016/j.bbrc.2017.08.123.
Murphy S, Patrick K, Thoner T, Edwards RW and Gubbels Bupp MR. 2016. T cell up-regulation of CD127 is associated with reductions in the homeostatic set point of the peripheral Tcell pool during malnourishment. Biochemistry and Biophysics Reports. doi:10.1016/j.bbrep.2016.06.006
Barwick BG, Scharer CD, Bally APR and Boss JM. 2016. Plasma Cell Differentiation is Coupled to Division-Dependent DNA Hypomethylation and Gene Regulation. Nat Immunol. 17(10):1216-25
| Name | CFSE |
|---|---|
| Cat. No. | 13-0850 |
| Protocol | CFSE Labeling Protocol |
| Excitation Laser | Blue (488 nm) |
| Emission (nm) | 521 |
| Formulation | lyophilized (500 ug) |
| Application | Flow Cytometry |
Product Information
Product Information
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Description
CFSE is a non-fluorescent molecule that easily diffuses across cell membranes. Inside the cell, acetate groups are cleaved by intracellular esterases yielding a fluorescent molecule whose succinimidyl ester group covalently interacts with primary amines of intracellular proteins. CFSE is compatible with standard intracellular staining protocols using aldehyde fixation and saponin-based permeabilization. CFSE is widely used to track cell division and to monitor cell migration in vivo. CFSE can be detected using standard fluorescein filter sets by fluorescence microscopy or flow cytometry.
Download the CFSE Labeling Protocol.
Recent Publications:
Meng YM, Jiang X, Zhao X, Meng Q, Wu S, Chen Y, Kong X, Qiu X, Su L, Huang C, Wang M, Liu C, Wong PP. Hexokinase 2-driven glycolysis in pericytes activates their contractility leading to tumor blood vessel abnormalities. Nat Commun. 2021 Oct 14;12(1):6011. doi: 10.1038/s41467-021-26259-y. PMID: 34650057.
» View on Nature Communications
Sun Y, Lu D, Yin Y, Song J, Liu Y, Hao W, Qi F, Zhang G, Zhang X, Liu L, Lin Z, Liang H, Zhao X, Jin Y, Yin Y. PTENα functions as an immune suppressor and promotes immune resistance in PTEN-mutant cancer. Nat Commun. 2021 Aug 26;12(1):5147. doi: 10.1038/s41467-021-25417-6. PMID: 34446716.
» View on Nature Communications
Do P, Perdue LA, Chyong A, Hunter R, Dougan J, Henry CJ, Porter CC, Dreaden EC. Rapid Assembly and Screening of Multivalent Immune Cell-Redirecting Therapies for Leukemia. ACS Comb Sci. 2020 Oct 12;22(10):533-541. doi: 10.1021/acscombsci.0c00081. Epub 2020 Aug 18. PMID: 32786324.
DomĂnguez-Romero AN, MartĂnez-CortĂ©s F, MunguĂa ME, Odales J, Gevorkian G, Manoutcharian K. Generation of multiepitope cancer vaccines based on large combinatorial libraries of survivin-derived mutant epitopes [published online ahead of print, 2020 Jul 3]. Immunology. 2020;10.1111/imm.13233. doi:10.1111/imm.13233.
Brincks E. L., Adams J., Wang L., Turner B., Marcinowicz A., Ke J., Essmann M., Mautino L. M., Allen C. Van, Kumar S., Vahanian N., Link C., Mautino M. R. et al Indoximod opposes the immunosuppressive effects mediated by IDO and TDO via modulation of AhR function and activation of mTORC1. Oncotarget. 2020; 11: 2438-2461.
Rana PS, Mudrak NJ, Lopez R, Lynn M, Kershner L and Model MA. 2017. Phase separation in necrotic cells. Biochem Biophys Res Commun. doi: 10.1016/j.bbrc.2017.08.123.
Murphy S, Patrick K, Thoner T, Edwards RW and Gubbels Bupp MR. 2016. T cell up-regulation of CD127 is associated with reductions in the homeostatic set point of the peripheral Tcell pool during malnourishment. Biochemistry and Biophysics Reports. doi:10.1016/j.bbrep.2016.06.006
Barwick BG, Scharer CD, Bally APR and Boss JM. 2016. Plasma Cell Differentiation is Coupled to Division-Dependent DNA Hypomethylation and Gene Regulation. Nat Immunol. 17(10):1216-25
| Name | CFSE |
|---|---|
| Cat. No. | 13-0850 |
| Protocol | CFSE Labeling Protocol |
| Excitation Laser | Blue (488 nm) |
| Emission (nm) | 521 |
| Formulation | lyophilized (500 ug) |
| Application | Flow Cytometry |
