V5 Tag Monoclonal Antibody (TCM5), PE-Cyanine7, eBioscience
PRODUCT DETAILS
Host: Mouse
Isotype: IgG2b, kappa
Clonality: Monoclonal
Clone: TCM5
Format: PE-Cyanine7
Reactivity: Tag
Application: Flow Cytometry
Tested Dilution: 5 µL (0.06 µg)/test
Concentration: 5 μL/Test
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: This TCM5 monoclonal antibody recognizes recombinant proteins containing a V5 epitope tag. The V5 epitope is naturally present on the P and V proteins of the paramyxovirus, SV5. TCM5 specifically recognizes the sequence GKPIPNPLLGLDST.
Applications Reported: This TCM5 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This TCM5 antibody has been pre-diluted and tested by flow cytometric analysis of the human 293T cancer cell line transfected with the V5 tagged Type II transmembrane protein CD207 and a GFP reporter gene. This may be used at 5 µL (0.06 µg) per test for surface staining and at 1.25 µL (0.015 µg) per test for intracellular staining. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.Original: $336.00
-70%$336.00
$100.80V5 Tag Monoclonal Antibody (TCM5), PE-Cyanine7, eBioscience
PRODUCT DETAILS
Host: Mouse
Isotype: IgG2b, kappa
Clonality: Monoclonal
Clone: TCM5
Format: PE-Cyanine7
Reactivity: Tag
Application: Flow Cytometry
Tested Dilution: 5 µL (0.06 µg)/test
Concentration: 5 μL/Test
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: This TCM5 monoclonal antibody recognizes recombinant proteins containing a V5 epitope tag. The V5 epitope is naturally present on the P and V proteins of the paramyxovirus, SV5. TCM5 specifically recognizes the sequence GKPIPNPLLGLDST.
Applications Reported: This TCM5 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This TCM5 antibody has been pre-diluted and tested by flow cytometric analysis of the human 293T cancer cell line transfected with the V5 tagged Type II transmembrane protein CD207 and a GFP reporter gene. This may be used at 5 µL (0.06 µg) per test for surface staining and at 1.25 µL (0.015 µg) per test for intracellular staining. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Â
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.Product Information
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Description
PRODUCT DETAILS
Host: Mouse
Isotype: IgG2b, kappa
Clonality: Monoclonal
Clone: TCM5
Format: PE-Cyanine7
Reactivity: Tag
Application: Flow Cytometry
Tested Dilution: 5 µL (0.06 µg)/test
Concentration: 5 μL/Test
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: This TCM5 monoclonal antibody recognizes recombinant proteins containing a V5 epitope tag. The V5 epitope is naturally present on the P and V proteins of the paramyxovirus, SV5. TCM5 specifically recognizes the sequence GKPIPNPLLGLDST.
Applications Reported: This TCM5 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This TCM5 antibody has been pre-diluted and tested by flow cytometric analysis of the human 293T cancer cell line transfected with the V5 tagged Type II transmembrane protein CD207 and a GFP reporter gene. This may be used at 5 µL (0.06 µg) per test for surface staining and at 1.25 µL (0.015 µg) per test for intracellular staining. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
