TCR V beta F1 Monoclonal Antibody (8A3), PE-Cyanine7, eBioscience
PRODUCT DETAILS
Host: Mouse
Isotype: IgG1, kappa
Clonality: Monoclonal
Clone: 8A3
Format: PE-Cyanine7
Reactivity: Hu
Application: Flow Cytometry
Tested Dilution: 5 µL (0.125 µg)/test
Concentration: 5 μL/Test
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
This 8A3 monoclonal antibody recognizes human TCR beta F1, a common framework determinant on the beta-subunit of the T cell receptor. Expression of TCR beta F1 has been described as a potential diagnostic marker indicating a favorable outcome for tumor patients with not otherwise specified Nodal Peripheral T-cell Lymphoma (PTCL-NOS). Furthermore, a lack of surface and cytoplasmic TCR beta F1 was also found in small cell variant of T-cell prolymphocytic Leukemia. This antibody will not stain the native form of the TCR and will require fixing and permeabilizing cells.
Applications Reported: This 8A3 antibody has been reported for use in flow cytometric analysis, immunofluorescent staining of frozen tissue sections, and immunocytochemistry.
Applications Tested: This 8A3 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-34) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.Original: $452.00
-70%$452.00
$135.60TCR V beta F1 Monoclonal Antibody (8A3), PE-Cyanine7, eBioscience
PRODUCT DETAILS
Host: Mouse
Isotype: IgG1, kappa
Clonality: Monoclonal
Clone: 8A3
Format: PE-Cyanine7
Reactivity: Hu
Application: Flow Cytometry
Tested Dilution: 5 µL (0.125 µg)/test
Concentration: 5 μL/Test
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
This 8A3 monoclonal antibody recognizes human TCR beta F1, a common framework determinant on the beta-subunit of the T cell receptor. Expression of TCR beta F1 has been described as a potential diagnostic marker indicating a favorable outcome for tumor patients with not otherwise specified Nodal Peripheral T-cell Lymphoma (PTCL-NOS). Furthermore, a lack of surface and cytoplasmic TCR beta F1 was also found in small cell variant of T-cell prolymphocytic Leukemia. This antibody will not stain the native form of the TCR and will require fixing and permeabilizing cells.
Applications Reported: This 8A3 antibody has been reported for use in flow cytometric analysis, immunofluorescent staining of frozen tissue sections, and immunocytochemistry.
Applications Tested: This 8A3 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-34) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.Product Information
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Description
PRODUCT DETAILS
Host: Mouse
Isotype: IgG1, kappa
Clonality: Monoclonal
Clone: 8A3
Format: PE-Cyanine7
Reactivity: Hu
Application: Flow Cytometry
Tested Dilution: 5 µL (0.125 µg)/test
Concentration: 5 μL/Test
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
This 8A3 monoclonal antibody recognizes human TCR beta F1, a common framework determinant on the beta-subunit of the T cell receptor. Expression of TCR beta F1 has been described as a potential diagnostic marker indicating a favorable outcome for tumor patients with not otherwise specified Nodal Peripheral T-cell Lymphoma (PTCL-NOS). Furthermore, a lack of surface and cytoplasmic TCR beta F1 was also found in small cell variant of T-cell prolymphocytic Leukemia. This antibody will not stain the native form of the TCR and will require fixing and permeabilizing cells.
Applications Reported: This 8A3 antibody has been reported for use in flow cytometric analysis, immunofluorescent staining of frozen tissue sections, and immunocytochemistry.
Applications Tested: This 8A3 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-34) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
