TCR V beta 12 Monoclonal Antibody (MR11-1), PerCP-eFluor 710, eBioscience
PRODUCT DETAILS
Host: Mouse
Isotype: IgG1, kappa
Clonality: Monoclonal
Clone: MR11-1
Format: PerCP-eFluor 710
Reactivity: Ms
Application: Flow Cytometry
Tested Dilution: 0.25 µg/test
Concentration: 0.2 mg/mL
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: This MR11-1 monoclonal antibody reacts with the mouse T cell receptor (TCR) V beta 12 chain. Composed of an alpha and beta chain, TCR specificity is typically determined by Va, Ja, Vb, Db, and Jb gene rearrangement. The MR11-1 antibody recognizes the V beta 12 chain on T cells from mouse strains with the b haplotype, including C57BL/6, B10, and C58/J. V beta 12+ T cells are absent in strains with the a (e.g., SJL) or c (e.g., RIII) haplotypes of the Tcrb gene complex. Finally, mouse strains expressing the minor lymphocyte stimulatory (Mls) antigens, such as BALB/c and C3H, express fewer V beta 12+ T cells.
Applications Reported: This MR11-1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This MR11-1 antibody has been tested by flow cytometric analysis of mouse lymph node. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.
Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.Original: $156.00
-70%$156.00
$46.80TCR V beta 12 Monoclonal Antibody (MR11-1), PerCP-eFluor 710, eBioscience
PRODUCT DETAILS
Host: Mouse
Isotype: IgG1, kappa
Clonality: Monoclonal
Clone: MR11-1
Format: PerCP-eFluor 710
Reactivity: Ms
Application: Flow Cytometry
Tested Dilution: 0.25 µg/test
Concentration: 0.2 mg/mL
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: This MR11-1 monoclonal antibody reacts with the mouse T cell receptor (TCR) V beta 12 chain. Composed of an alpha and beta chain, TCR specificity is typically determined by Va, Ja, Vb, Db, and Jb gene rearrangement. The MR11-1 antibody recognizes the V beta 12 chain on T cells from mouse strains with the b haplotype, including C57BL/6, B10, and C58/J. V beta 12+ T cells are absent in strains with the a (e.g., SJL) or c (e.g., RIII) haplotypes of the Tcrb gene complex. Finally, mouse strains expressing the minor lymphocyte stimulatory (Mls) antigens, such as BALB/c and C3H, express fewer V beta 12+ T cells.
Applications Reported: This MR11-1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This MR11-1 antibody has been tested by flow cytometric analysis of mouse lymph node. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.
Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.Product Information
Product Information
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Description
PRODUCT DETAILS
Host: Mouse
Isotype: IgG1, kappa
Clonality: Monoclonal
Clone: MR11-1
Format: PerCP-eFluor 710
Reactivity: Ms
Application: Flow Cytometry
Tested Dilution: 0.25 µg/test
Concentration: 0.2 mg/mL
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: This MR11-1 monoclonal antibody reacts with the mouse T cell receptor (TCR) V beta 12 chain. Composed of an alpha and beta chain, TCR specificity is typically determined by Va, Ja, Vb, Db, and Jb gene rearrangement. The MR11-1 antibody recognizes the V beta 12 chain on T cells from mouse strains with the b haplotype, including C57BL/6, B10, and C58/J. V beta 12+ T cells are absent in strains with the a (e.g., SJL) or c (e.g., RIII) haplotypes of the Tcrb gene complex. Finally, mouse strains expressing the minor lymphocyte stimulatory (Mls) antigens, such as BALB/c and C3H, express fewer V beta 12+ T cells.
Applications Reported: This MR11-1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This MR11-1 antibody has been tested by flow cytometric analysis of mouse lymph node. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.
Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
