SSEA3 Monoclonal Antibody (eBioMC-631 (MC-631)), eBioscience

PRODUCT DETAILS

Host: Rat

Isotype: IgM

Clonality: Monoclonal

Clone: eBioMC-631 (MC-631)

Reactivity: Hu, Ms

Application: Flow Cytometry

Tested Dilution: 0.5 µg/test

Concentration: 0.5 mg/mL

Storage: 4°C

Formulation: PBS with 0.09% sodium azide; pH 7.2

Purification: Affinity chromatography

Data Sheet: TDS

Specific Information

Description: The eBioMC-631 (MC-631) monoclonal antibody reacts with the carbohydrate moiety galactosylgloboside on Stage-specific embryonic antigen-3 (SSEA-3). Expression is restricted to the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES) and down regulated following differentiation.

Applications Reported: This eBioMC-631 (MC-631) antibody has been reported for use in flow cytometric analysis, immunohistochemical staining, and immunocytochemistry.

Applications Tested: This eBioMC-631 (MC-631) antibody has been tested by flow cytometric analysis of the 2102Ep cell line. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

 

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.