Snail1 Monoclonal Antibody (20C8), Alexa Fluor 488, eBioscience
PRODUCT DETAILS
Host: Mouse
Isotype: IgG2a, kappa
Clonality: Monoclonal
Clone: 20C8
Format: Alexa Fluor 488
Reactivity: Hu
Application: Flow Cytometry
Tested Dilution: Assay-Dependent
Concentration: 0.5 mg/mL
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The monoclonal antibody 20C8 recognizes human Snail1, a 29 kDa zinc finger transcription factor. Snail1 and Slug both belong to a family of transcription factors that are responsible for inducing epithelial to mesenchymal transition (EMT) during normal organ development and tumor progression. Snail1 expression is localized to both the cytoplasm and nucleus and functions as a transcriptional repressor of E-cadherin, claudins, occludin, and desmoplakin. During embryonic development, Snail1 is localized to the mesoderm, while in the adult, expression is inducible and is usually limited to activated fibroblasts and some mesenchymal cells. Overexpression of Snail1 has been found in carcinomas and fibrosarcomas and may be used as an early marker for EMT.
Applications Reported: This 20C8 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded (IHC-P) tissue, ELISA, immunoprecipitation, western blotting, immunocytochemistry, and intracellular staining followed by flow cytometric analysis.
Applications Tested: This 20C8 antibody has been tested by immunocytochemistry on fixed and permeabilized MCF7 cells and by immunohistochemistry on formalin-fixed paraffin embedded human infiltrating ductal carcinoma. This antibody can be used at less than or equal to 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.Snail1 Monoclonal Antibody (20C8), Alexa Fluor 488, eBioscience
PRODUCT DETAILS
Host: Mouse
Isotype: IgG2a, kappa
Clonality: Monoclonal
Clone: 20C8
Format: Alexa Fluor 488
Reactivity: Hu
Application: Flow Cytometry
Tested Dilution: Assay-Dependent
Concentration: 0.5 mg/mL
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The monoclonal antibody 20C8 recognizes human Snail1, a 29 kDa zinc finger transcription factor. Snail1 and Slug both belong to a family of transcription factors that are responsible for inducing epithelial to mesenchymal transition (EMT) during normal organ development and tumor progression. Snail1 expression is localized to both the cytoplasm and nucleus and functions as a transcriptional repressor of E-cadherin, claudins, occludin, and desmoplakin. During embryonic development, Snail1 is localized to the mesoderm, while in the adult, expression is inducible and is usually limited to activated fibroblasts and some mesenchymal cells. Overexpression of Snail1 has been found in carcinomas and fibrosarcomas and may be used as an early marker for EMT.
Applications Reported: This 20C8 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded (IHC-P) tissue, ELISA, immunoprecipitation, western blotting, immunocytochemistry, and intracellular staining followed by flow cytometric analysis.
Applications Tested: This 20C8 antibody has been tested by immunocytochemistry on fixed and permeabilized MCF7 cells and by immunohistochemistry on formalin-fixed paraffin embedded human infiltrating ductal carcinoma. This antibody can be used at less than or equal to 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Â
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.Product Information
Product Information
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Description
PRODUCT DETAILS
Host: Mouse
Isotype: IgG2a, kappa
Clonality: Monoclonal
Clone: 20C8
Format: Alexa Fluor 488
Reactivity: Hu
Application: Flow Cytometry
Tested Dilution: Assay-Dependent
Concentration: 0.5 mg/mL
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The monoclonal antibody 20C8 recognizes human Snail1, a 29 kDa zinc finger transcription factor. Snail1 and Slug both belong to a family of transcription factors that are responsible for inducing epithelial to mesenchymal transition (EMT) during normal organ development and tumor progression. Snail1 expression is localized to both the cytoplasm and nucleus and functions as a transcriptional repressor of E-cadherin, claudins, occludin, and desmoplakin. During embryonic development, Snail1 is localized to the mesoderm, while in the adult, expression is inducible and is usually limited to activated fibroblasts and some mesenchymal cells. Overexpression of Snail1 has been found in carcinomas and fibrosarcomas and may be used as an early marker for EMT.
Applications Reported: This 20C8 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded (IHC-P) tissue, ELISA, immunoprecipitation, western blotting, immunocytochemistry, and intracellular staining followed by flow cytometric analysis.
Applications Tested: This 20C8 antibody has been tested by immunocytochemistry on fixed and permeabilized MCF7 cells and by immunohistochemistry on formalin-fixed paraffin embedded human infiltrating ductal carcinoma. This antibody can be used at less than or equal to 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Â
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
