Myeloperoxidase (MPO) Monoclonal Antibody (MPO455-8E6), eFluor 450, eBioscience
PRODUCT DETAILS
Host: Mouse
Isotype: IgG1
Clonality: Monoclonal
Clone: MPO455-8E6
Format: eFluor 450
Reactivity: Hu
Application: Flow Cytometry
Tested Dilution: 5 µL (0.25 µg)/test
Concentration: 5 μL/Test
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The monoclonal antibody MPO455-8E6 recognizes myeloperoxidase (MPO), a protein within the azurophilic granules of myeloid cells. MPO is a multimeric protein comprised of two 55 kDa subunits and two 15 kDa subunits. The larger subunits associate with a heme protein resulting in a greenish color. As an enzyme, MPO breaks down hydrogen peroxide and oxidizes tyrosine. The products of this reaction, hypochlorous acid and tyrosyl radical, causethe cytotoxic and killing effects characteristic of neutrophils. Myeloperoxidase is important in the diagnosis of some cancers and increases in serum levels have been shown to correlate with cardiac events.
Applications Reported: This MPO455-8E6 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This MPO455-8E6 antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells using the Intracellular Fixation & Permeablization Buffer Set (Product # 88-8824-00). For best results, whole blood should first be stained with antibodies to surface antigens then treated with 1X RBC lysis buffer (Product # 00-4333-57) to lyse erythrocytes. Then, for intracellular staining for MPO, cells should be fixed with IC Fixation Buffer (Product # 00-8222-49) washed two times with Permeabilization Buffer (Product # 00-8333-56) and then incubated with MPO455-8E6 for 30-60 minutes. After washing, cells may be analyzed on a flow cytometer. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.
Filtration: 0.2 µm post-manufacturing filtered.
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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.Original: $414.00
-70%$414.00
$124.20Myeloperoxidase (MPO) Monoclonal Antibody (MPO455-8E6), eFluor 450, eBioscience
PRODUCT DETAILS
Host: Mouse
Isotype: IgG1
Clonality: Monoclonal
Clone: MPO455-8E6
Format: eFluor 450
Reactivity: Hu
Application: Flow Cytometry
Tested Dilution: 5 µL (0.25 µg)/test
Concentration: 5 μL/Test
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The monoclonal antibody MPO455-8E6 recognizes myeloperoxidase (MPO), a protein within the azurophilic granules of myeloid cells. MPO is a multimeric protein comprised of two 55 kDa subunits and two 15 kDa subunits. The larger subunits associate with a heme protein resulting in a greenish color. As an enzyme, MPO breaks down hydrogen peroxide and oxidizes tyrosine. The products of this reaction, hypochlorous acid and tyrosyl radical, causethe cytotoxic and killing effects characteristic of neutrophils. Myeloperoxidase is important in the diagnosis of some cancers and increases in serum levels have been shown to correlate with cardiac events.
Applications Reported: This MPO455-8E6 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This MPO455-8E6 antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells using the Intracellular Fixation & Permeablization Buffer Set (Product # 88-8824-00). For best results, whole blood should first be stained with antibodies to surface antigens then treated with 1X RBC lysis buffer (Product # 00-4333-57) to lyse erythrocytes. Then, for intracellular staining for MPO, cells should be fixed with IC Fixation Buffer (Product # 00-8222-49) washed two times with Permeabilization Buffer (Product # 00-8333-56) and then incubated with MPO455-8E6 for 30-60 minutes. After washing, cells may be analyzed on a flow cytometer. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Â
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.Product Information
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Description
PRODUCT DETAILS
Host: Mouse
Isotype: IgG1
Clonality: Monoclonal
Clone: MPO455-8E6
Format: eFluor 450
Reactivity: Hu
Application: Flow Cytometry
Tested Dilution: 5 µL (0.25 µg)/test
Concentration: 5 μL/Test
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The monoclonal antibody MPO455-8E6 recognizes myeloperoxidase (MPO), a protein within the azurophilic granules of myeloid cells. MPO is a multimeric protein comprised of two 55 kDa subunits and two 15 kDa subunits. The larger subunits associate with a heme protein resulting in a greenish color. As an enzyme, MPO breaks down hydrogen peroxide and oxidizes tyrosine. The products of this reaction, hypochlorous acid and tyrosyl radical, causethe cytotoxic and killing effects characteristic of neutrophils. Myeloperoxidase is important in the diagnosis of some cancers and increases in serum levels have been shown to correlate with cardiac events.
Applications Reported: This MPO455-8E6 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This MPO455-8E6 antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells using the Intracellular Fixation & Permeablization Buffer Set (Product # 88-8824-00). For best results, whole blood should first be stained with antibodies to surface antigens then treated with 1X RBC lysis buffer (Product # 00-4333-57) to lyse erythrocytes. Then, for intracellular staining for MPO, cells should be fixed with IC Fixation Buffer (Product # 00-8222-49) washed two times with Permeabilization Buffer (Product # 00-8333-56) and then incubated with MPO455-8E6 for 30-60 minutes. After washing, cells may be analyzed on a flow cytometer. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Â
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
