GFP Monoclonal Antibody (5F12.4), eFluor 660, eBioscience
PRODUCT DETAILS
Host: Rat
Isotype: IgG2a, kappa
Clonality: Monoclonal
Clone: 5F12.4
Format: eFluor 660
Reactivity: Tag
Application: Flow Cytometry
Tested Dilution: 0.125 µg/test
Concentration: 0.2 mg/mL
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: This 5F12.4 monoclonal antibody reacts with green-fluorescent protein (GFP), which was originally isolated from the cnidarian Aequorea victoria. This protein absorbs blue light (maximally at 395 nm) and emits green light (peak at 509) without the requirement of exogenous substrates and cofactors. These unique qualities allow GFP to be used to monitor gene expression and protein localization in vivo. Several mutant forms of GFP have been developed which fluoresce more intensely and have shifted excitation maxima when compared to wildtype GFP, making them useful for flow cytometry, fluorescence microscopy, and double-labeling applications. This antibody is capable of staining formaldehyde fixed cells.
Applications Reported: This 5F12.4 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 5F12.4 antibody has been tested by flow cytometric analysis of GFP expressing cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
eFluorâ„¢ 660 is a replacement for Alexa Fluorâ„¢ 647. eFluorâ„¢ 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.Original: $532.00
-70%$532.00
$159.60GFP Monoclonal Antibody (5F12.4), eFluor 660, eBioscience
PRODUCT DETAILS
Host: Rat
Isotype: IgG2a, kappa
Clonality: Monoclonal
Clone: 5F12.4
Format: eFluor 660
Reactivity: Tag
Application: Flow Cytometry
Tested Dilution: 0.125 µg/test
Concentration: 0.2 mg/mL
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: This 5F12.4 monoclonal antibody reacts with green-fluorescent protein (GFP), which was originally isolated from the cnidarian Aequorea victoria. This protein absorbs blue light (maximally at 395 nm) and emits green light (peak at 509) without the requirement of exogenous substrates and cofactors. These unique qualities allow GFP to be used to monitor gene expression and protein localization in vivo. Several mutant forms of GFP have been developed which fluoresce more intensely and have shifted excitation maxima when compared to wildtype GFP, making them useful for flow cytometry, fluorescence microscopy, and double-labeling applications. This antibody is capable of staining formaldehyde fixed cells.
Applications Reported: This 5F12.4 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 5F12.4 antibody has been tested by flow cytometric analysis of GFP expressing cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
eFluorâ„¢ 660 is a replacement for Alexa Fluorâ„¢ 647. eFluorâ„¢ 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Â
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.Product Information
Product Information
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Shipping & Returns
Description
PRODUCT DETAILS
Host: Rat
Isotype: IgG2a, kappa
Clonality: Monoclonal
Clone: 5F12.4
Format: eFluor 660
Reactivity: Tag
Application: Flow Cytometry
Tested Dilution: 0.125 µg/test
Concentration: 0.2 mg/mL
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: This 5F12.4 monoclonal antibody reacts with green-fluorescent protein (GFP), which was originally isolated from the cnidarian Aequorea victoria. This protein absorbs blue light (maximally at 395 nm) and emits green light (peak at 509) without the requirement of exogenous substrates and cofactors. These unique qualities allow GFP to be used to monitor gene expression and protein localization in vivo. Several mutant forms of GFP have been developed which fluoresce more intensely and have shifted excitation maxima when compared to wildtype GFP, making them useful for flow cytometry, fluorescence microscopy, and double-labeling applications. This antibody is capable of staining formaldehyde fixed cells.
Applications Reported: This 5F12.4 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 5F12.4 antibody has been tested by flow cytometric analysis of GFP expressing cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
eFluorâ„¢ 660 is a replacement for Alexa Fluorâ„¢ 647. eFluorâ„¢ 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Â
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
