Cytochrome C Monoclonal Antibody (2B5), FITC, eBioscience
PRODUCT DETAILS
Host: Mouse
Isotype: IgG2a
Clonality: Monoclonal
Clone: 2B5
Format: FITC
Reactivity: Hu
Application: Flow Cytometry
Tested Dilution: Assay-Dependent
Concentration: 0.1 mg/mL
Storage: 4°C, store in dark
Formulation: TBS with 1% BSA and 0.02% sodium azide; pH 7.2-7.4
Purification: Protein G
Data Sheet: TDS
Specific Information
Description: Cytochrome c was identified as a component required for the crucial steps in apoptosis, caspase-3 activation and DNA fragmentation. Cytochrome c was shown to redistribute from mitochondria to cytosol during apoptosis in intact cells.
Mitochrondrial cytochrome c is a water-soluble protein of 15 kDa with a net positive charge, residing loosely attached in the mitochrondrial intermembrane space. Cytochrome c functions in the respiratory chain by interaction with redox partners. The release of cytochrome c into the cytosol leads to an activation of an apoptotic program via activation of a caspase dependent pathway. Cytochrome c achieves this goal by interaction with other cytosolic factors forming a complex (apoptosome) composed of cytochrome c, Apaf-1, dATP and Apaf-3/caspase 9. Bcl-2 on the other hand was shown to be able to prevent apoptosis by blocking the release of cytochrome c from mitochondria.
Applications Tested: Flow Cytometry.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser
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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.Cytochrome C Monoclonal Antibody (2B5), FITC, eBioscience
PRODUCT DETAILS
Host: Mouse
Isotype: IgG2a
Clonality: Monoclonal
Clone: 2B5
Format: FITC
Reactivity: Hu
Application: Flow Cytometry
Tested Dilution: Assay-Dependent
Concentration: 0.1 mg/mL
Storage: 4°C, store in dark
Formulation: TBS with 1% BSA and 0.02% sodium azide; pH 7.2-7.4
Purification: Protein G
Data Sheet: TDS
Specific Information
Description: Cytochrome c was identified as a component required for the crucial steps in apoptosis, caspase-3 activation and DNA fragmentation. Cytochrome c was shown to redistribute from mitochondria to cytosol during apoptosis in intact cells.
Mitochrondrial cytochrome c is a water-soluble protein of 15 kDa with a net positive charge, residing loosely attached in the mitochrondrial intermembrane space. Cytochrome c functions in the respiratory chain by interaction with redox partners. The release of cytochrome c into the cytosol leads to an activation of an apoptotic program via activation of a caspase dependent pathway. Cytochrome c achieves this goal by interaction with other cytosolic factors forming a complex (apoptosome) composed of cytochrome c, Apaf-1, dATP and Apaf-3/caspase 9. Bcl-2 on the other hand was shown to be able to prevent apoptosis by blocking the release of cytochrome c from mitochondria.
Applications Tested: Flow Cytometry.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser
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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.Product Information
Product Information
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Description
PRODUCT DETAILS
Host: Mouse
Isotype: IgG2a
Clonality: Monoclonal
Clone: 2B5
Format: FITC
Reactivity: Hu
Application: Flow Cytometry
Tested Dilution: Assay-Dependent
Concentration: 0.1 mg/mL
Storage: 4°C, store in dark
Formulation: TBS with 1% BSA and 0.02% sodium azide; pH 7.2-7.4
Purification: Protein G
Data Sheet: TDS
Specific Information
Description: Cytochrome c was identified as a component required for the crucial steps in apoptosis, caspase-3 activation and DNA fragmentation. Cytochrome c was shown to redistribute from mitochondria to cytosol during apoptosis in intact cells.
Mitochrondrial cytochrome c is a water-soluble protein of 15 kDa with a net positive charge, residing loosely attached in the mitochrondrial intermembrane space. Cytochrome c functions in the respiratory chain by interaction with redox partners. The release of cytochrome c into the cytosol leads to an activation of an apoptotic program via activation of a caspase dependent pathway. Cytochrome c achieves this goal by interaction with other cytosolic factors forming a complex (apoptosome) composed of cytochrome c, Apaf-1, dATP and Apaf-3/caspase 9. Bcl-2 on the other hand was shown to be able to prevent apoptosis by blocking the release of cytochrome c from mitochondria.
Applications Tested: Flow Cytometry.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser
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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
