CD8a Monoclonal Antibody (YCATE55.9), Super Bright 436, eBioscience
PRODUCT DETAILS
Host: Rat
Isotype: IgG1, kappa
Clonality: Monoclonal
Clone: YCATE55.9
Format: Super Bright 436
Reactivity: Ca
Application: Flow Cytometry
Tested Dilution: 5 µL (1.0 µg)/test
Concentration: 5 μL/Test
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: This YCATE55.9 monoclonal antibody reacts with canine CD8 alpha. This cell surface receptor is expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). In dogs, CD8 alpha is expressed on thymocytes, a subset of mature T cells, NK cells, and intestinal intraepithelial lymphocytes (IELs). CD8 binds to MHC class I and plays a role in thymocyte development and mature T cell activation.
This monoclonal antibody has been reported to inhibit cytotoxic T cell generation and function in vitro. In addition, this antibody has been reported to be immunosuppressive in vivo.
Applications Reported: This YCATE55.9 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This YCATE55.9 antibody has been pre-titrated and tested by flow cytometric analysis of normal canine peripheral blood cells. This can be used at 5 µL (1.0 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.
When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.Original: $407.00
-70%$407.00
$122.10CD8a Monoclonal Antibody (YCATE55.9), Super Bright 436, eBioscience
PRODUCT DETAILS
Host: Rat
Isotype: IgG1, kappa
Clonality: Monoclonal
Clone: YCATE55.9
Format: Super Bright 436
Reactivity: Ca
Application: Flow Cytometry
Tested Dilution: 5 µL (1.0 µg)/test
Concentration: 5 μL/Test
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: This YCATE55.9 monoclonal antibody reacts with canine CD8 alpha. This cell surface receptor is expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). In dogs, CD8 alpha is expressed on thymocytes, a subset of mature T cells, NK cells, and intestinal intraepithelial lymphocytes (IELs). CD8 binds to MHC class I and plays a role in thymocyte development and mature T cell activation.
This monoclonal antibody has been reported to inhibit cytotoxic T cell generation and function in vitro. In addition, this antibody has been reported to be immunosuppressive in vivo.
Applications Reported: This YCATE55.9 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This YCATE55.9 antibody has been pre-titrated and tested by flow cytometric analysis of normal canine peripheral blood cells. This can be used at 5 µL (1.0 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.
When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Â
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.Product Information
Product Information
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Description
PRODUCT DETAILS
Host: Rat
Isotype: IgG1, kappa
Clonality: Monoclonal
Clone: YCATE55.9
Format: Super Bright 436
Reactivity: Ca
Application: Flow Cytometry
Tested Dilution: 5 µL (1.0 µg)/test
Concentration: 5 μL/Test
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: This YCATE55.9 monoclonal antibody reacts with canine CD8 alpha. This cell surface receptor is expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). In dogs, CD8 alpha is expressed on thymocytes, a subset of mature T cells, NK cells, and intestinal intraepithelial lymphocytes (IELs). CD8 binds to MHC class I and plays a role in thymocyte development and mature T cell activation.
This monoclonal antibody has been reported to inhibit cytotoxic T cell generation and function in vitro. In addition, this antibody has been reported to be immunosuppressive in vivo.
Applications Reported: This YCATE55.9 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This YCATE55.9 antibody has been pre-titrated and tested by flow cytometric analysis of normal canine peripheral blood cells. This can be used at 5 µL (1.0 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.
When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Â
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
