CD58 (LFA-3) Monoclonal Antibody (TS2/9), PerCP-eFluor 710, eBioscience
PRODUCT DETAILS
Host: Mouse
Isotype: IgG1, kappa
Clonality: Monoclonal
Clone: TS2/9
Format: PerCP-eFluor 710
Reactivity: Hu
Application: Flow Cytometry
Tested Dilution: 5 µL (0.5 µg)/test
Concentration: 5 μL/Test
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The TS2/9 antibody recognizes human CD58, which is also known as Lymphocyte Associated Antigen-3 (LFA-3). CD58 exists both as a transmembrane protein as well as a GPI-linked extracellular protein. It has been shown that cells can express both forms concurrently. In addition, soluble CD58 has also been observed in serum, urine and synoval fluid of rheumatoid arthritis patients. CD58 is found on non-hematopoietic and most hematopoietic cells. Binding to its ligand CD2, which is present on T cells, initiates a signaling pathway which includes phosphorylation and results in release of cytokines. CD58 has been shown to be important in adhesion to various cell types, T cell activation, cytokine production and cytoxicity.
The TS2/9 antibody has been reported to induce signaling events upon crosslinking. The epitope is in domain 1 of the immunoglobulin region.
Applications Reported: This TS2/9 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This TS2/9 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
PerCP-eFluorâ„¢ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.Original: $445.00
-70%$445.00
$133.50CD58 (LFA-3) Monoclonal Antibody (TS2/9), PerCP-eFluor 710, eBioscience
PRODUCT DETAILS
Host: Mouse
Isotype: IgG1, kappa
Clonality: Monoclonal
Clone: TS2/9
Format: PerCP-eFluor 710
Reactivity: Hu
Application: Flow Cytometry
Tested Dilution: 5 µL (0.5 µg)/test
Concentration: 5 μL/Test
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The TS2/9 antibody recognizes human CD58, which is also known as Lymphocyte Associated Antigen-3 (LFA-3). CD58 exists both as a transmembrane protein as well as a GPI-linked extracellular protein. It has been shown that cells can express both forms concurrently. In addition, soluble CD58 has also been observed in serum, urine and synoval fluid of rheumatoid arthritis patients. CD58 is found on non-hematopoietic and most hematopoietic cells. Binding to its ligand CD2, which is present on T cells, initiates a signaling pathway which includes phosphorylation and results in release of cytokines. CD58 has been shown to be important in adhesion to various cell types, T cell activation, cytokine production and cytoxicity.
The TS2/9 antibody has been reported to induce signaling events upon crosslinking. The epitope is in domain 1 of the immunoglobulin region.
Applications Reported: This TS2/9 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This TS2/9 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
PerCP-eFluorâ„¢ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.Product Information
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Description
PRODUCT DETAILS
Host: Mouse
Isotype: IgG1, kappa
Clonality: Monoclonal
Clone: TS2/9
Format: PerCP-eFluor 710
Reactivity: Hu
Application: Flow Cytometry
Tested Dilution: 5 µL (0.5 µg)/test
Concentration: 5 μL/Test
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The TS2/9 antibody recognizes human CD58, which is also known as Lymphocyte Associated Antigen-3 (LFA-3). CD58 exists both as a transmembrane protein as well as a GPI-linked extracellular protein. It has been shown that cells can express both forms concurrently. In addition, soluble CD58 has also been observed in serum, urine and synoval fluid of rheumatoid arthritis patients. CD58 is found on non-hematopoietic and most hematopoietic cells. Binding to its ligand CD2, which is present on T cells, initiates a signaling pathway which includes phosphorylation and results in release of cytokines. CD58 has been shown to be important in adhesion to various cell types, T cell activation, cytokine production and cytoxicity.
The TS2/9 antibody has been reported to induce signaling events upon crosslinking. The epitope is in domain 1 of the immunoglobulin region.
Applications Reported: This TS2/9 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This TS2/9 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
PerCP-eFluorâ„¢ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
