CD49b (Integrin alpha 2) Monoclonal Antibody (eBioY418 (Y418)), eBioscience

PRODUCT DETAILS

Host: Mouse

Isotype: IgG1, kappa

Clonality: Monoclonal

Clone: eBioY418 (Y418)

Reactivity: Hu

Application: Flow Cytometry

Tested Dilution: 0.25 µg/test

Concentration: 0.5 mg/mL

Storage: 4°C

Formulation: PBS with 0.09% sodium azide; pH 7.2

Purification: Affinity chromatography

Data Sheet: TDS

Specific Information

Description: The eBioY418 (Y418) monoclonal antibody reacts with human CD49b, the 150-kDa, integrin alpha 2 subunit. The complex of CD49b non-covalently associated with integrin beta 1 (CD29), also known as VLA-2, is a receptor for collagen and laminin. This complex is expressed by lymphocytes, activated T cells, monocytes and platelets.

Applications Reported: This eBioY418 (Y418) antibody has been reported for use in flow cytometric analysis. (Fluorochrome-conjugated eBioY418 is recommended for use in flow cytometry.).

Applications Tested: This eBioY418 (Y418) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

 

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.