CD45R (B220) Monoclonal Antibody (RA3-6B2), eFluor 506, eBioscience
PRODUCT DETAILS
Host: Rat
Isotype: IgG2a, kappa
Clonality: Monoclonal
Clone: RA3-6B2
Format: eFluor 506
Reactivity: Hu, Ms
Application: Flow Cytometry
Tested Dilution: 0.5 µg/test
Concentration: 0.2 mg/mL
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The RA3-6B2 monoclonal antibody reacts with exon A-restricted isoform of mouse CD45, a 220 kDa surface molecule. CD45R/B220 epitope is mainly expressed by the B cell lineage from early Pro-B to mature B cells. However, some activated T cells, lymphokine activated killer cells (LAK), NK cell progenitors in the bone marrow, and T cells of the lpr/lpr mutant mouse also express this antigen.
Applications Reported: This RA3-6B2 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This RA3-6B2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
eFluor® 506 can be excited with the violet laser line (405 nm) and emits at 506 nm. We recommend using a 510/20 band pass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.
Excitation: 405 nm; Emission: 506 nm; Laser: Violet Laser.
Filtration: 0.2 µm post-manufacturing filtered.
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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.CD45R (B220) Monoclonal Antibody (RA3-6B2), eFluor 506, eBioscience
PRODUCT DETAILS
Host: Rat
Isotype: IgG2a, kappa
Clonality: Monoclonal
Clone: RA3-6B2
Format: eFluor 506
Reactivity: Hu, Ms
Application: Flow Cytometry
Tested Dilution: 0.5 µg/test
Concentration: 0.2 mg/mL
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The RA3-6B2 monoclonal antibody reacts with exon A-restricted isoform of mouse CD45, a 220 kDa surface molecule. CD45R/B220 epitope is mainly expressed by the B cell lineage from early Pro-B to mature B cells. However, some activated T cells, lymphokine activated killer cells (LAK), NK cell progenitors in the bone marrow, and T cells of the lpr/lpr mutant mouse also express this antigen.
Applications Reported: This RA3-6B2 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This RA3-6B2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
eFluor® 506 can be excited with the violet laser line (405 nm) and emits at 506 nm. We recommend using a 510/20 band pass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.
Excitation: 405 nm; Emission: 506 nm; Laser: Violet Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Â
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.Product Information
Product Information
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Description
PRODUCT DETAILS
Host: Rat
Isotype: IgG2a, kappa
Clonality: Monoclonal
Clone: RA3-6B2
Format: eFluor 506
Reactivity: Hu, Ms
Application: Flow Cytometry
Tested Dilution: 0.5 µg/test
Concentration: 0.2 mg/mL
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The RA3-6B2 monoclonal antibody reacts with exon A-restricted isoform of mouse CD45, a 220 kDa surface molecule. CD45R/B220 epitope is mainly expressed by the B cell lineage from early Pro-B to mature B cells. However, some activated T cells, lymphokine activated killer cells (LAK), NK cell progenitors in the bone marrow, and T cells of the lpr/lpr mutant mouse also express this antigen.
Applications Reported: This RA3-6B2 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This RA3-6B2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
eFluor® 506 can be excited with the violet laser line (405 nm) and emits at 506 nm. We recommend using a 510/20 band pass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.
Excitation: 405 nm; Emission: 506 nm; Laser: Violet Laser.
Filtration: 0.2 µm post-manufacturing filtered.
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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
