CD45 Monoclonal Antibody (30-F11), eBioscience
PRODUCT DETAILS
Host: Rat
Isotype: IgG2b, kappa
Clonality: Monoclonal
Clone: 30-F11
Reactivity: Ms
Application: Flow Cytometry
Tested Dilution: 0.5 µg/test
Concentration: 0.5 mg/mL
Storage: 4°C
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The 30-F11 monoclonal antibody reacts with all isoforms of mouse CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding mature erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.
Applications Reported: The 30-F11 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining. 30-F11 has also been reported in complement-dependent cytotoxicity. (Please use Functional Grade purified 30-F11 in functional assays).
Applications Tested: The 30-F11 antibody has been tested by flow cytometric analysis of mouse bone marrow cells and splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.CD45 Monoclonal Antibody (30-F11), eBioscience
PRODUCT DETAILS
Host: Rat
Isotype: IgG2b, kappa
Clonality: Monoclonal
Clone: 30-F11
Reactivity: Ms
Application: Flow Cytometry
Tested Dilution: 0.5 µg/test
Concentration: 0.5 mg/mL
Storage: 4°C
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The 30-F11 monoclonal antibody reacts with all isoforms of mouse CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding mature erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.
Applications Reported: The 30-F11 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining. 30-F11 has also been reported in complement-dependent cytotoxicity. (Please use Functional Grade purified 30-F11 in functional assays).
Applications Tested: The 30-F11 antibody has been tested by flow cytometric analysis of mouse bone marrow cells and splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Â
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.Product Information
Product Information
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Description
PRODUCT DETAILS
Host: Rat
Isotype: IgG2b, kappa
Clonality: Monoclonal
Clone: 30-F11
Reactivity: Ms
Application: Flow Cytometry
Tested Dilution: 0.5 µg/test
Concentration: 0.5 mg/mL
Storage: 4°C
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The 30-F11 monoclonal antibody reacts with all isoforms of mouse CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding mature erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.
Applications Reported: The 30-F11 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining. 30-F11 has also been reported in complement-dependent cytotoxicity. (Please use Functional Grade purified 30-F11 in functional assays).
Applications Tested: The 30-F11 antibody has been tested by flow cytometric analysis of mouse bone marrow cells and splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Â
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
