CD3e Monoclonal Antibody (145-2C11), Functional Grade, eBioscience
PRODUCT DETAILS
Host: Armenian Hamster
Isotype: IgG
Clonality: Monoclonal
Clone: 145-2C11
Format: Functional Grade
Reactivity: Ms
Application: Flow Cytometry
Tested Dilution: 0.5 µg/test
Concentration: 1 mg/mL
Storage: 4°C
Formulation: PBS with no preservative; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The 145-2C11 monoclonal antibody reacts with mouse CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits, gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 145-2C11 to TCR initiates the intracellular biochemical pathway resulting in cellular activation, proliferation, and apoptosis depending on specific conditions utilized. 145-2C11 is commonly used as a phenotypic marker for mouse T cells.
Applications Reported:The 145-2C11 antibody has been reported for use in flow cytometric analysis. It has also been reported in cell activation and cell depletion. Please visit the BestProtocols® webpage to view a protocol for in vitro T-cell activation.
Applications Tested: The 145-2C11 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level:Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation:Less than 10%, as determined by HPLC.
Â
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.Original: $162.00
-70%$162.00
$48.60CD3e Monoclonal Antibody (145-2C11), Functional Grade, eBioscience
PRODUCT DETAILS
Host: Armenian Hamster
Isotype: IgG
Clonality: Monoclonal
Clone: 145-2C11
Format: Functional Grade
Reactivity: Ms
Application: Flow Cytometry
Tested Dilution: 0.5 µg/test
Concentration: 1 mg/mL
Storage: 4°C
Formulation: PBS with no preservative; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The 145-2C11 monoclonal antibody reacts with mouse CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits, gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 145-2C11 to TCR initiates the intracellular biochemical pathway resulting in cellular activation, proliferation, and apoptosis depending on specific conditions utilized. 145-2C11 is commonly used as a phenotypic marker for mouse T cells.
Applications Reported:The 145-2C11 antibody has been reported for use in flow cytometric analysis. It has also been reported in cell activation and cell depletion. Please visit the BestProtocols® webpage to view a protocol for in vitro T-cell activation.
Applications Tested: The 145-2C11 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level:Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation:Less than 10%, as determined by HPLC.
Â
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.Product Information
Product Information
Shipping & Returns
Shipping & Returns
Description
PRODUCT DETAILS
Host: Armenian Hamster
Isotype: IgG
Clonality: Monoclonal
Clone: 145-2C11
Format: Functional Grade
Reactivity: Ms
Application: Flow Cytometry
Tested Dilution: 0.5 µg/test
Concentration: 1 mg/mL
Storage: 4°C
Formulation: PBS with no preservative; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The 145-2C11 monoclonal antibody reacts with mouse CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits, gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 145-2C11 to TCR initiates the intracellular biochemical pathway resulting in cellular activation, proliferation, and apoptosis depending on specific conditions utilized. 145-2C11 is commonly used as a phenotypic marker for mouse T cells.
Applications Reported:The 145-2C11 antibody has been reported for use in flow cytometric analysis. It has also been reported in cell activation and cell depletion. Please visit the BestProtocols® webpage to view a protocol for in vitro T-cell activation.
Applications Tested: The 145-2C11 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level:Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation:Less than 10%, as determined by HPLC.
Â
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
