CD3 Monoclonal Antibody (OKT3), eBioscience
PRODUCT DETAILS
Host: Mouse
Isotype: IgG2a, kappa
Clonality: Monoclonal
Clone: OKT3
Reactivity: Hu
Application: Flow Cytometry
Tested Dilution: 0.25 µg/test
Concentration: 0.5 mg/mL
Storage: 4°C
Formulation: TBS with 0.09% sodium azide; pH 8.0
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex. The OKT3 antibody has been reported to have potent immunosuppressive properties in vivo and has been proven effective in the treatment of renal, heart and liver allograft rejection. The CD3 subunits, gamma, delta, and epsilon chains, are required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR initiates an intracellular biochemical pathway resulting in cellular activation and proliferation.
Applications Reported: The OKT3 antibody has been reported for use in flow cytometric analysis. OKT3 has also been reported in in vitro activation of T cells. (Please use Functional Grade purified OKT3, Product # 16-0037, in functional assays).
Applications Tested: The OKT3 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.Original: $173.00
-70%$173.00
$51.90CD3 Monoclonal Antibody (OKT3), eBioscience
PRODUCT DETAILS
Host: Mouse
Isotype: IgG2a, kappa
Clonality: Monoclonal
Clone: OKT3
Reactivity: Hu
Application: Flow Cytometry
Tested Dilution: 0.25 µg/test
Concentration: 0.5 mg/mL
Storage: 4°C
Formulation: TBS with 0.09% sodium azide; pH 8.0
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex. The OKT3 antibody has been reported to have potent immunosuppressive properties in vivo and has been proven effective in the treatment of renal, heart and liver allograft rejection. The CD3 subunits, gamma, delta, and epsilon chains, are required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR initiates an intracellular biochemical pathway resulting in cellular activation and proliferation.
Applications Reported: The OKT3 antibody has been reported for use in flow cytometric analysis. OKT3 has also been reported in in vitro activation of T cells. (Please use Functional Grade purified OKT3, Product # 16-0037, in functional assays).
Applications Tested: The OKT3 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Â
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.Product Information
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Description
PRODUCT DETAILS
Host: Mouse
Isotype: IgG2a, kappa
Clonality: Monoclonal
Clone: OKT3
Reactivity: Hu
Application: Flow Cytometry
Tested Dilution: 0.25 µg/test
Concentration: 0.5 mg/mL
Storage: 4°C
Formulation: TBS with 0.09% sodium azide; pH 8.0
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex. The OKT3 antibody has been reported to have potent immunosuppressive properties in vivo and has been proven effective in the treatment of renal, heart and liver allograft rejection. The CD3 subunits, gamma, delta, and epsilon chains, are required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR initiates an intracellular biochemical pathway resulting in cellular activation and proliferation.
Applications Reported: The OKT3 antibody has been reported for use in flow cytometric analysis. OKT3 has also been reported in in vitro activation of T cells. (Please use Functional Grade purified OKT3, Product # 16-0037, in functional assays).
Applications Tested: The OKT3 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
