CD3/CD4/CD8a Antibody Cocktail, APC, FITC, PerCP-eFluor 710, eBioscience
PRODUCT DETAILS
Host: Mouse
Isotype: IgG1, kappa
Clonality: Cocktail
Clone: SK3, SK7, SK1
Format: APC/FITC/PerCP-eFluor 710
Reactivity: Hu
Application: Flow Cytometry
Tested Dilution: 20 µL/test
Concentration: 20 μL/Test
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: This three-color cocktail is designed to identify T cells from peripheral blood samples. It can be used alone, or in combination with other antibodies to further characterize T cell populations. The SK3 monoclonal antibody reacts with human CD4, a 59 kDa cell surface receptor expressed by a majority of thymocytes, a subpopulation of mature T helper cells, and in low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). The SK7 monoclonal antibody reacts with human CD3 eplison, a 20 kDa subunit of the CD3/TCR complex. The CD3 complex, including the epsilon, gamma and delta subunits, is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. The SK1 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8beta chain (CD8alpha beta) or as a homodimer (CD8alphaalpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a.
This cocktail contains the following antibodies:
Anti-Human CD4 (SK3) FITC
Anti-Human CD3 (SK7) PerCP-eFluorâ„¢ 710
Anti-Human CD8a (SK1) APC
Applications Reported: eZfluor Anti-Human CD4 FITC, CD3 PerCP-eFluorâ„¢ 710, CD8a APC has been reported for use in flow cytometric analysis.
Applications Tested: eZfluor Anti-Human CD4 FITC, CD3 PerCP-eFluor™ 710, CD8a APC has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 20 µL per test, which contains 0.06 µg of Anti-Human CD4 FITC, 0.06 µg of Anti-Human CD3 PerCP-eFluor™ 710, and 0.25 µg of Anti-Human CD8a APC. A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.CD3/CD4/CD8a Antibody Cocktail, APC, FITC, PerCP-eFluor 710, eBioscience
PRODUCT DETAILS
Host: Mouse
Isotype: IgG1, kappa
Clonality: Cocktail
Clone: SK3, SK7, SK1
Format: APC/FITC/PerCP-eFluor 710
Reactivity: Hu
Application: Flow Cytometry
Tested Dilution: 20 µL/test
Concentration: 20 μL/Test
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: This three-color cocktail is designed to identify T cells from peripheral blood samples. It can be used alone, or in combination with other antibodies to further characterize T cell populations. The SK3 monoclonal antibody reacts with human CD4, a 59 kDa cell surface receptor expressed by a majority of thymocytes, a subpopulation of mature T helper cells, and in low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). The SK7 monoclonal antibody reacts with human CD3 eplison, a 20 kDa subunit of the CD3/TCR complex. The CD3 complex, including the epsilon, gamma and delta subunits, is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. The SK1 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8beta chain (CD8alpha beta) or as a homodimer (CD8alphaalpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a.
This cocktail contains the following antibodies:
Anti-Human CD4 (SK3) FITC
Anti-Human CD3 (SK7) PerCP-eFluorâ„¢ 710
Anti-Human CD8a (SK1) APC
Applications Reported: eZfluor Anti-Human CD4 FITC, CD3 PerCP-eFluorâ„¢ 710, CD8a APC has been reported for use in flow cytometric analysis.
Applications Tested: eZfluor Anti-Human CD4 FITC, CD3 PerCP-eFluor™ 710, CD8a APC has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 20 µL per test, which contains 0.06 µg of Anti-Human CD4 FITC, 0.06 µg of Anti-Human CD3 PerCP-eFluor™ 710, and 0.25 µg of Anti-Human CD8a APC. A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.Product Information
Product Information
Shipping & Returns
Shipping & Returns
Description
PRODUCT DETAILS
Host: Mouse
Isotype: IgG1, kappa
Clonality: Cocktail
Clone: SK3, SK7, SK1
Format: APC/FITC/PerCP-eFluor 710
Reactivity: Hu
Application: Flow Cytometry
Tested Dilution: 20 µL/test
Concentration: 20 μL/Test
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: This three-color cocktail is designed to identify T cells from peripheral blood samples. It can be used alone, or in combination with other antibodies to further characterize T cell populations. The SK3 monoclonal antibody reacts with human CD4, a 59 kDa cell surface receptor expressed by a majority of thymocytes, a subpopulation of mature T helper cells, and in low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). The SK7 monoclonal antibody reacts with human CD3 eplison, a 20 kDa subunit of the CD3/TCR complex. The CD3 complex, including the epsilon, gamma and delta subunits, is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. The SK1 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8beta chain (CD8alpha beta) or as a homodimer (CD8alphaalpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a.
This cocktail contains the following antibodies:
Anti-Human CD4 (SK3) FITC
Anti-Human CD3 (SK7) PerCP-eFluorâ„¢ 710
Anti-Human CD8a (SK1) APC
Applications Reported: eZfluor Anti-Human CD4 FITC, CD3 PerCP-eFluorâ„¢ 710, CD8a APC has been reported for use in flow cytometric analysis.
Applications Tested: eZfluor Anti-Human CD4 FITC, CD3 PerCP-eFluor™ 710, CD8a APC has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 20 µL per test, which contains 0.06 µg of Anti-Human CD4 FITC, 0.06 µg of Anti-Human CD3 PerCP-eFluor™ 710, and 0.25 µg of Anti-Human CD8a APC. A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
