CD276 (B7-H3) Monoclonal Antibody (MIH35), Functional Grade, eBioscience
PRODUCT DETAILS
Host: Rat
Isotype: IgG2a, kappa
Clonality: Monoclonal
Clone: MIH35
Format: Functional Grade
Reactivity: Hu, Ms
Application: Flow Cytometry
Tested Dilution: 1 µg/test
Concentration: 1 mg/mL
Storage: 4°C
Formulation: PBS with no preservative; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: This monoclonal antibody reacts with mouse and human CD276 (also known as B7-H3), a type I transmembrane protein belonging to the B7 family of costimulatory proteins. B7-H3 is expressed weakly on activated lymphocytes, macrophages, dendritic cells, nasal and airway epithelial cells, osteoblasts, and some tumor cell lines. A soluble form of B7-H3 is also secreted by monocytes, dendritic cells, and activated T cells. Although the biological role of this molecule remains controversial, recent studies have identified B7-H3 as the ligand for TREM-like 2 (TLT-2).
Applications Reported: This MIH35 antibody has been reported for use in functional assays and flow cytometric analysis.
Applications Tested: This MIH35 antibody has been tested for blocking activity in mouse, as well as by flow cytometric analysis on transfected cell lines. This can be used at 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.CD276 (B7-H3) Monoclonal Antibody (MIH35), Functional Grade, eBioscience
PRODUCT DETAILS
Host: Rat
Isotype: IgG2a, kappa
Clonality: Monoclonal
Clone: MIH35
Format: Functional Grade
Reactivity: Hu, Ms
Application: Flow Cytometry
Tested Dilution: 1 µg/test
Concentration: 1 mg/mL
Storage: 4°C
Formulation: PBS with no preservative; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: This monoclonal antibody reacts with mouse and human CD276 (also known as B7-H3), a type I transmembrane protein belonging to the B7 family of costimulatory proteins. B7-H3 is expressed weakly on activated lymphocytes, macrophages, dendritic cells, nasal and airway epithelial cells, osteoblasts, and some tumor cell lines. A soluble form of B7-H3 is also secreted by monocytes, dendritic cells, and activated T cells. Although the biological role of this molecule remains controversial, recent studies have identified B7-H3 as the ligand for TREM-like 2 (TLT-2).
Applications Reported: This MIH35 antibody has been reported for use in functional assays and flow cytometric analysis.
Applications Tested: This MIH35 antibody has been tested for blocking activity in mouse, as well as by flow cytometric analysis on transfected cell lines. This can be used at 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.Product Information
Product Information
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Description
PRODUCT DETAILS
Host: Rat
Isotype: IgG2a, kappa
Clonality: Monoclonal
Clone: MIH35
Format: Functional Grade
Reactivity: Hu, Ms
Application: Flow Cytometry
Tested Dilution: 1 µg/test
Concentration: 1 mg/mL
Storage: 4°C
Formulation: PBS with no preservative; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: This monoclonal antibody reacts with mouse and human CD276 (also known as B7-H3), a type I transmembrane protein belonging to the B7 family of costimulatory proteins. B7-H3 is expressed weakly on activated lymphocytes, macrophages, dendritic cells, nasal and airway epithelial cells, osteoblasts, and some tumor cell lines. A soluble form of B7-H3 is also secreted by monocytes, dendritic cells, and activated T cells. Although the biological role of this molecule remains controversial, recent studies have identified B7-H3 as the ligand for TREM-like 2 (TLT-2).
Applications Reported: This MIH35 antibody has been reported for use in functional assays and flow cytometric analysis.
Applications Tested: This MIH35 antibody has been tested for blocking activity in mouse, as well as by flow cytometric analysis on transfected cell lines. This can be used at 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
