CD195 (CCR5) Monoclonal Antibody (eBioT21/8 (T21/8)), eBioscience
PRODUCT DETAILS
Host: Mouse
Isotype: IgG1, kappa
Clonality: Monoclonal
Clone: eBioT21/8 (T21/8)
Reactivity: Hu
Application: Flow Cytometry
Tested Dilution: 0.5 µg/test
Concentration: 0.5 mg/mL
Storage: 4°C
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The eBioT21/8 monoclonal antibody reacts with the amino terminus of human CCR5 (CD195), an approximately 45 kDa member of the G protein-coupled receptor (GPCR) superfamily that binds chemokines including MIP-1alpha, MIP-1beta and RANTES. Human CCR5 is expressed on resting T cells with a memory/effector phenotype, monocytes, macrophages and immature dendritic cells. CCR5 functions as a receptor to direct the chemotaxis of lymphocytes, and is the primary co-receptor for macrophage-tropic Human Immunodeficiency Virus Type 1 (HIV-1), which binds CCR5 through gp120. Binding of the eBioT21/8 monoclonal antibody displays donor variability and may be affected by sub-optimal staining conditions.
Applications Reported: This eBioT21/8 (T21/8) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB) and immunocytochemical staining. The eBioT21/8 monoclonal antibody has also been used as the capture antibody for the ELISA detection of human CCR5 in cell lysates.
Applications Tested: This eBioT21/8 (T21/8) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. For optimal performance, it is recommended that staining with NP-6G4 be done at 37°C for 1 hour in Flow Cytometry Staining Buffer (Product # 00-4222-57). This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Binding of the eBioT21/8 monoclonal antibody displays donor variability and may be affected by sup-optimal staining conditions. Therefore, staining conditions should be closely monitored to ensure consistent staining.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.CD195 (CCR5) Monoclonal Antibody (eBioT21/8 (T21/8)), eBioscience
PRODUCT DETAILS
Host: Mouse
Isotype: IgG1, kappa
Clonality: Monoclonal
Clone: eBioT21/8 (T21/8)
Reactivity: Hu
Application: Flow Cytometry
Tested Dilution: 0.5 µg/test
Concentration: 0.5 mg/mL
Storage: 4°C
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The eBioT21/8 monoclonal antibody reacts with the amino terminus of human CCR5 (CD195), an approximately 45 kDa member of the G protein-coupled receptor (GPCR) superfamily that binds chemokines including MIP-1alpha, MIP-1beta and RANTES. Human CCR5 is expressed on resting T cells with a memory/effector phenotype, monocytes, macrophages and immature dendritic cells. CCR5 functions as a receptor to direct the chemotaxis of lymphocytes, and is the primary co-receptor for macrophage-tropic Human Immunodeficiency Virus Type 1 (HIV-1), which binds CCR5 through gp120. Binding of the eBioT21/8 monoclonal antibody displays donor variability and may be affected by sub-optimal staining conditions.
Applications Reported: This eBioT21/8 (T21/8) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB) and immunocytochemical staining. The eBioT21/8 monoclonal antibody has also been used as the capture antibody for the ELISA detection of human CCR5 in cell lysates.
Applications Tested: This eBioT21/8 (T21/8) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. For optimal performance, it is recommended that staining with NP-6G4 be done at 37°C for 1 hour in Flow Cytometry Staining Buffer (Product # 00-4222-57). This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Binding of the eBioT21/8 monoclonal antibody displays donor variability and may be affected by sup-optimal staining conditions. Therefore, staining conditions should be closely monitored to ensure consistent staining.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.Product Information
Product Information
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Description
PRODUCT DETAILS
Host: Mouse
Isotype: IgG1, kappa
Clonality: Monoclonal
Clone: eBioT21/8 (T21/8)
Reactivity: Hu
Application: Flow Cytometry
Tested Dilution: 0.5 µg/test
Concentration: 0.5 mg/mL
Storage: 4°C
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The eBioT21/8 monoclonal antibody reacts with the amino terminus of human CCR5 (CD195), an approximately 45 kDa member of the G protein-coupled receptor (GPCR) superfamily that binds chemokines including MIP-1alpha, MIP-1beta and RANTES. Human CCR5 is expressed on resting T cells with a memory/effector phenotype, monocytes, macrophages and immature dendritic cells. CCR5 functions as a receptor to direct the chemotaxis of lymphocytes, and is the primary co-receptor for macrophage-tropic Human Immunodeficiency Virus Type 1 (HIV-1), which binds CCR5 through gp120. Binding of the eBioT21/8 monoclonal antibody displays donor variability and may be affected by sub-optimal staining conditions.
Applications Reported: This eBioT21/8 (T21/8) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB) and immunocytochemical staining. The eBioT21/8 monoclonal antibody has also been used as the capture antibody for the ELISA detection of human CCR5 in cell lysates.
Applications Tested: This eBioT21/8 (T21/8) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. For optimal performance, it is recommended that staining with NP-6G4 be done at 37°C for 1 hour in Flow Cytometry Staining Buffer (Product # 00-4222-57). This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Binding of the eBioT21/8 monoclonal antibody displays donor variability and may be affected by sup-optimal staining conditions. Therefore, staining conditions should be closely monitored to ensure consistent staining.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
