CD154 (CD40 Ligand) Monoclonal Antibody (24-31), eBioscience
PRODUCT DETAILS
Host: Mouse
Isotype: IgG1, kappa
Clonality: Monoclonal
Clone: 24-31
Reactivity: Cy, Hu
Application: Flow Cytometry
Tested Dilution: 1 µg/test
Concentration: 0.5 mg/mL
Storage: 4°C
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The 24-31 monoclonal antibody reacts with human CD154, a 39 kDa transmembrane glycoprotein also known as gp39 and CD40 ligand (CD40L). CD154 is a member of the TNF superfamily and is expressed transiently by activated T cells. Through its binding to CD40 on antigen presenting cells including B cells, monocytes/macrophages and dendritic cells, CD154 serves a crucial function in T-APC cognate interaction. CD154 interaction with CD40 transduces signals for T-dependent B-cell activation and induces B cell cycle entry. 24-31 cross-reacts with the cynomolgus monkey CD154.
Applications Reported: 24-31 has been reported for use in flow cytometric analysis. 24-31 has also been reported to block CD154 binding to CD40 and inhibit T cell-dependent B cell differentiation and antibody response in vitro. (Please use Functional Grade Purified 24-31, Product # 16-1548, in functional assays).
Applications Tested: The 24-31 antibody has been tested by flow cytometric analysis of resting and activated human normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.Original: $288.00
-70%$288.00
$86.40CD154 (CD40 Ligand) Monoclonal Antibody (24-31), eBioscience
PRODUCT DETAILS
Host: Mouse
Isotype: IgG1, kappa
Clonality: Monoclonal
Clone: 24-31
Reactivity: Cy, Hu
Application: Flow Cytometry
Tested Dilution: 1 µg/test
Concentration: 0.5 mg/mL
Storage: 4°C
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The 24-31 monoclonal antibody reacts with human CD154, a 39 kDa transmembrane glycoprotein also known as gp39 and CD40 ligand (CD40L). CD154 is a member of the TNF superfamily and is expressed transiently by activated T cells. Through its binding to CD40 on antigen presenting cells including B cells, monocytes/macrophages and dendritic cells, CD154 serves a crucial function in T-APC cognate interaction. CD154 interaction with CD40 transduces signals for T-dependent B-cell activation and induces B cell cycle entry. 24-31 cross-reacts with the cynomolgus monkey CD154.
Applications Reported: 24-31 has been reported for use in flow cytometric analysis. 24-31 has also been reported to block CD154 binding to CD40 and inhibit T cell-dependent B cell differentiation and antibody response in vitro. (Please use Functional Grade Purified 24-31, Product # 16-1548, in functional assays).
Applications Tested: The 24-31 antibody has been tested by flow cytometric analysis of resting and activated human normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Â
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.Product Information
Product Information
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Description
PRODUCT DETAILS
Host: Mouse
Isotype: IgG1, kappa
Clonality: Monoclonal
Clone: 24-31
Reactivity: Cy, Hu
Application: Flow Cytometry
Tested Dilution: 1 µg/test
Concentration: 0.5 mg/mL
Storage: 4°C
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The 24-31 monoclonal antibody reacts with human CD154, a 39 kDa transmembrane glycoprotein also known as gp39 and CD40 ligand (CD40L). CD154 is a member of the TNF superfamily and is expressed transiently by activated T cells. Through its binding to CD40 on antigen presenting cells including B cells, monocytes/macrophages and dendritic cells, CD154 serves a crucial function in T-APC cognate interaction. CD154 interaction with CD40 transduces signals for T-dependent B-cell activation and induces B cell cycle entry. 24-31 cross-reacts with the cynomolgus monkey CD154.
Applications Reported: 24-31 has been reported for use in flow cytometric analysis. 24-31 has also been reported to block CD154 binding to CD40 and inhibit T cell-dependent B cell differentiation and antibody response in vitro. (Please use Functional Grade Purified 24-31, Product # 16-1548, in functional assays).
Applications Tested: The 24-31 antibody has been tested by flow cytometric analysis of resting and activated human normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
