CD107a (LAMP-1) Monoclonal Antibody (eBio1D4B (1D4B)), APC-eFluor 780, eBioscience
PRODUCT DETAILS
Host: Rat
Isotype: IgG2a, kappa
Clonality: Monoclonal
Clone: eBio1D4B (1D4B)
Format: APC-eFluor 780
Reactivity: Ms
Application: Flow Cytometry
Tested Dilution: 1.0 µg/test
Concentration: 0.2 mg/mL
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The eBio1D4B monoclonal antibody reacts with mouse CD107a, also known as lysosomal-associated membrane protein-1 (LAMP-1). CD107a is a type I, lysosomal membrane protein that is extensively glycosylated. It is expressed constitutively in the late endosomes-lysosomes in all cells. CD107a is also transiently expressed on the cell surface of degranulating cytolytic T cells. Additionally, CD107a has been implicated in a variety of cellular functions including cancer metastasis and is also a marker for lysosomal storage disorders.
Applications Reported: This eBio1D4B antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This eBio1D4B antibody has been tested by by intracellular staining followed by flow cytometric analysis of thioglycolate-elicited peritoneal macrophages using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol A: Two step protocol for intracellular (cytoplasmic) proteins" located at Flow Protocols . This may be used at less than or equal to 1.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer ((Product # 00-8222-49)) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution ((Product # 00-5333-57)) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.CD107a (LAMP-1) Monoclonal Antibody (eBio1D4B (1D4B)), APC-eFluor 780, eBioscience
PRODUCT DETAILS
Host: Rat
Isotype: IgG2a, kappa
Clonality: Monoclonal
Clone: eBio1D4B (1D4B)
Format: APC-eFluor 780
Reactivity: Ms
Application: Flow Cytometry
Tested Dilution: 1.0 µg/test
Concentration: 0.2 mg/mL
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The eBio1D4B monoclonal antibody reacts with mouse CD107a, also known as lysosomal-associated membrane protein-1 (LAMP-1). CD107a is a type I, lysosomal membrane protein that is extensively glycosylated. It is expressed constitutively in the late endosomes-lysosomes in all cells. CD107a is also transiently expressed on the cell surface of degranulating cytolytic T cells. Additionally, CD107a has been implicated in a variety of cellular functions including cancer metastasis and is also a marker for lysosomal storage disorders.
Applications Reported: This eBio1D4B antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This eBio1D4B antibody has been tested by by intracellular staining followed by flow cytometric analysis of thioglycolate-elicited peritoneal macrophages using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol A: Two step protocol for intracellular (cytoplasmic) proteins" located at Flow Protocols . This may be used at less than or equal to 1.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer ((Product # 00-8222-49)) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution ((Product # 00-5333-57)) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.Product Information
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Description
PRODUCT DETAILS
Host: Rat
Isotype: IgG2a, kappa
Clonality: Monoclonal
Clone: eBio1D4B (1D4B)
Format: APC-eFluor 780
Reactivity: Ms
Application: Flow Cytometry
Tested Dilution: 1.0 µg/test
Concentration: 0.2 mg/mL
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The eBio1D4B monoclonal antibody reacts with mouse CD107a, also known as lysosomal-associated membrane protein-1 (LAMP-1). CD107a is a type I, lysosomal membrane protein that is extensively glycosylated. It is expressed constitutively in the late endosomes-lysosomes in all cells. CD107a is also transiently expressed on the cell surface of degranulating cytolytic T cells. Additionally, CD107a has been implicated in a variety of cellular functions including cancer metastasis and is also a marker for lysosomal storage disorders.
Applications Reported: This eBio1D4B antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This eBio1D4B antibody has been tested by by intracellular staining followed by flow cytometric analysis of thioglycolate-elicited peritoneal macrophages using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol A: Two step protocol for intracellular (cytoplasmic) proteins" located at Flow Protocols . This may be used at less than or equal to 1.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer ((Product # 00-8222-49)) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution ((Product # 00-5333-57)) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
