CD100 (SEMA4D) Monoclonal Antibody (BMA12 (BMA-12)), eBioscience
PRODUCT DETAILS
Host: Rat
Isotype: IgG2a
Clonality: Monoclonal
Clone: BMA12 (BMA-12)
Reactivity: Ms
Application: Flow Cytometry
Tested Dilution: 0.25 µg/test
Concentration: 0.5 mg/mL
Storage: 4°C
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The BMA12 monoclonal antibody reacts with mouse CD100, also known as Sema4D. Sema4D/CD100 is a 150kDa transmembrane protein expressed constitutively on T cells and is involved in the activation of B cells through its interaction with CD72. Interaction of these two proteins diminishes the negative signaling normally effected by CD72. CD100 is already expressed strongly on resting T cells and somewhat weakly on B cells, but activation upregulates expression on both cell types. Antigen expression has been reported in Spleen, thymus and lymph nodes.
Applications Reported: The BMA12 (BMA-12) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining. It has also been reported for use in neutralization of mouse CD100. (Please use Functional Grade purified BMA12, Product # 16-1001, in functional assays).
Applications Tested: The BMA12 (BMA-12) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.Original: $382.00
-70%$382.00
$114.60CD100 (SEMA4D) Monoclonal Antibody (BMA12 (BMA-12)), eBioscience
PRODUCT DETAILS
Host: Rat
Isotype: IgG2a
Clonality: Monoclonal
Clone: BMA12 (BMA-12)
Reactivity: Ms
Application: Flow Cytometry
Tested Dilution: 0.25 µg/test
Concentration: 0.5 mg/mL
Storage: 4°C
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The BMA12 monoclonal antibody reacts with mouse CD100, also known as Sema4D. Sema4D/CD100 is a 150kDa transmembrane protein expressed constitutively on T cells and is involved in the activation of B cells through its interaction with CD72. Interaction of these two proteins diminishes the negative signaling normally effected by CD72. CD100 is already expressed strongly on resting T cells and somewhat weakly on B cells, but activation upregulates expression on both cell types. Antigen expression has been reported in Spleen, thymus and lymph nodes.
Applications Reported: The BMA12 (BMA-12) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining. It has also been reported for use in neutralization of mouse CD100. (Please use Functional Grade purified BMA12, Product # 16-1001, in functional assays).
Applications Tested: The BMA12 (BMA-12) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Â
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.Product Information
Product Information
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Shipping & Returns
Description
PRODUCT DETAILS
Host: Rat
Isotype: IgG2a
Clonality: Monoclonal
Clone: BMA12 (BMA-12)
Reactivity: Ms
Application: Flow Cytometry
Tested Dilution: 0.25 µg/test
Concentration: 0.5 mg/mL
Storage: 4°C
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The BMA12 monoclonal antibody reacts with mouse CD100, also known as Sema4D. Sema4D/CD100 is a 150kDa transmembrane protein expressed constitutively on T cells and is involved in the activation of B cells through its interaction with CD72. Interaction of these two proteins diminishes the negative signaling normally effected by CD72. CD100 is already expressed strongly on resting T cells and somewhat weakly on B cells, but activation upregulates expression on both cell types. Antigen expression has been reported in Spleen, thymus and lymph nodes.
Applications Reported: The BMA12 (BMA-12) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining. It has also been reported for use in neutralization of mouse CD100. (Please use Functional Grade purified BMA12, Product # 16-1001, in functional assays).
Applications Tested: The BMA12 (BMA-12) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
