c-MAF Monoclonal Antibody (sym0F1), eFluor 450, eBioscience
PRODUCT DETAILS
Host: Mouse
Isotype: IgG2b, kappa
Clonality: Monoclonal
Clone: sym0F1
Format: eFluor 450
Reactivity: Hu, Ms
Application: Flow Cytometry
Tested Dilution: 5 µL (0.125 µg)/test
Concentration: 5 μL/Test
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The sym0F1 monoclonal antibody reacts with human and mouse c-Maf, a 42 kDa basic leucine zipper transcription factor shown to be involved in the neural, ocular and hematopoietic systems. In hematopoietic cells, it was first shown to be crucial for IL-4 expression in Th2 and was the first transcription factor believed to be Th subset-specific. More recent evidence shows that specific phospho-tyrosine residues lead to upregulation of IL-4. c-Maf has also been shown to be important to differentiation and function in both Th17 and Tfh cells. It drives expression of IL-21 in both cell types, while promoting expression of IL-23R in Th17 and CXCR5 in Tfh as well.
Applications Reported: This sym0F1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This sym0F1 antibody has been pre-diluted and tested by intracellular staining followed by flow cytometric analysis of Th17-polarized mouse splenocytes using the Foxp3/Transcription Factor Buffer Set and protocol. Please see BestProtocols® Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
eFluor 450 is a alternative to Pacific Blue.eFluor 450 emits at 445 nm and is excited with the violet laser line (405 nm). Please make sure that your instrument is capable of detecting this fluorochrome.
Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser
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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.Original: $499.00
-70%$499.00
$149.70c-MAF Monoclonal Antibody (sym0F1), eFluor 450, eBioscience
PRODUCT DETAILS
Host: Mouse
Isotype: IgG2b, kappa
Clonality: Monoclonal
Clone: sym0F1
Format: eFluor 450
Reactivity: Hu, Ms
Application: Flow Cytometry
Tested Dilution: 5 µL (0.125 µg)/test
Concentration: 5 μL/Test
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The sym0F1 monoclonal antibody reacts with human and mouse c-Maf, a 42 kDa basic leucine zipper transcription factor shown to be involved in the neural, ocular and hematopoietic systems. In hematopoietic cells, it was first shown to be crucial for IL-4 expression in Th2 and was the first transcription factor believed to be Th subset-specific. More recent evidence shows that specific phospho-tyrosine residues lead to upregulation of IL-4. c-Maf has also been shown to be important to differentiation and function in both Th17 and Tfh cells. It drives expression of IL-21 in both cell types, while promoting expression of IL-23R in Th17 and CXCR5 in Tfh as well.
Applications Reported: This sym0F1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This sym0F1 antibody has been pre-diluted and tested by intracellular staining followed by flow cytometric analysis of Th17-polarized mouse splenocytes using the Foxp3/Transcription Factor Buffer Set and protocol. Please see BestProtocols® Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
eFluor 450 is a alternative to Pacific Blue.eFluor 450 emits at 445 nm and is excited with the violet laser line (405 nm). Please make sure that your instrument is capable of detecting this fluorochrome.
Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser
Â
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.Product Information
Product Information
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Description
PRODUCT DETAILS
Host: Mouse
Isotype: IgG2b, kappa
Clonality: Monoclonal
Clone: sym0F1
Format: eFluor 450
Reactivity: Hu, Ms
Application: Flow Cytometry
Tested Dilution: 5 µL (0.125 µg)/test
Concentration: 5 μL/Test
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The sym0F1 monoclonal antibody reacts with human and mouse c-Maf, a 42 kDa basic leucine zipper transcription factor shown to be involved in the neural, ocular and hematopoietic systems. In hematopoietic cells, it was first shown to be crucial for IL-4 expression in Th2 and was the first transcription factor believed to be Th subset-specific. More recent evidence shows that specific phospho-tyrosine residues lead to upregulation of IL-4. c-Maf has also been shown to be important to differentiation and function in both Th17 and Tfh cells. It drives expression of IL-21 in both cell types, while promoting expression of IL-23R in Th17 and CXCR5 in Tfh as well.
Applications Reported: This sym0F1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This sym0F1 antibody has been pre-diluted and tested by intracellular staining followed by flow cytometric analysis of Th17-polarized mouse splenocytes using the Foxp3/Transcription Factor Buffer Set and protocol. Please see BestProtocols® Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
eFluor 450 is a alternative to Pacific Blue.eFluor 450 emits at 445 nm and is excited with the violet laser line (405 nm). Please make sure that your instrument is capable of detecting this fluorochrome.
Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser
Â
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
