Aiolos Monoclonal Antibody (8B2), eFluor 660, eBioscience
PRODUCT DETAILS
Host: Mouse
Isotype: IgG1, kappa
Clonality: Monoclonal
Clone: 8B2
Format: eFluor 660
Reactivity: Ms
Application: Flow Cytometry
Tested Dilution: 1 µg/test
Concentration: 0.2 mg/mL
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: This 8B2 monoclonal antibody reacts with mouse Aiolos, a 58-kDa member of the Ikaros family of zinc finger transcription factors. Expression of Aiolos is restricted to the lymphoid lineage, with B cells exhibiting the highest levels of expression, followed by NK and T cells. Studies demonstrate that Aiolos forms homodimers or heterodimerizes with Ikaros proteins. Moreover, Aiolos exhibits tyrosine phosphorylation and nuclear localization in response to IL-2 stimulation in T cells. Aiolos plays a role in IL-2-mediated anti-apoptosis by controlling Bcl-2 expression. Finally, Aiolos is involved in B cell development, with upregulation of this transcription factor observed at the pre-BI to pre-BII transition in response to pre-B cell receptor signaling.
Applications Reported: This 8B2 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This 8B2 antibody has been tested by intracellular staining and flow cytometric analysis of mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.Original: $530.00
-70%$530.00
$159.00Aiolos Monoclonal Antibody (8B2), eFluor 660, eBioscience
PRODUCT DETAILS
Host: Mouse
Isotype: IgG1, kappa
Clonality: Monoclonal
Clone: 8B2
Format: eFluor 660
Reactivity: Ms
Application: Flow Cytometry
Tested Dilution: 1 µg/test
Concentration: 0.2 mg/mL
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: This 8B2 monoclonal antibody reacts with mouse Aiolos, a 58-kDa member of the Ikaros family of zinc finger transcription factors. Expression of Aiolos is restricted to the lymphoid lineage, with B cells exhibiting the highest levels of expression, followed by NK and T cells. Studies demonstrate that Aiolos forms homodimers or heterodimerizes with Ikaros proteins. Moreover, Aiolos exhibits tyrosine phosphorylation and nuclear localization in response to IL-2 stimulation in T cells. Aiolos plays a role in IL-2-mediated anti-apoptosis by controlling Bcl-2 expression. Finally, Aiolos is involved in B cell development, with upregulation of this transcription factor observed at the pre-BI to pre-BII transition in response to pre-B cell receptor signaling.
Applications Reported: This 8B2 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This 8B2 antibody has been tested by intracellular staining and flow cytometric analysis of mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Â
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.Product Information
Product Information
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Description
PRODUCT DETAILS
Host: Mouse
Isotype: IgG1, kappa
Clonality: Monoclonal
Clone: 8B2
Format: eFluor 660
Reactivity: Ms
Application: Flow Cytometry
Tested Dilution: 1 µg/test
Concentration: 0.2 mg/mL
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: This 8B2 monoclonal antibody reacts with mouse Aiolos, a 58-kDa member of the Ikaros family of zinc finger transcription factors. Expression of Aiolos is restricted to the lymphoid lineage, with B cells exhibiting the highest levels of expression, followed by NK and T cells. Studies demonstrate that Aiolos forms homodimers or heterodimerizes with Ikaros proteins. Moreover, Aiolos exhibits tyrosine phosphorylation and nuclear localization in response to IL-2 stimulation in T cells. Aiolos plays a role in IL-2-mediated anti-apoptosis by controlling Bcl-2 expression. Finally, Aiolos is involved in B cell development, with upregulation of this transcription factor observed at the pre-BI to pre-BII transition in response to pre-B cell receptor signaling.
Applications Reported: This 8B2 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This 8B2 antibody has been tested by intracellular staining and flow cytometric analysis of mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Â
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
